Description
Boster’s mouse S100A9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for S100A9 has been precoated onto 96-well plates. Standards(E.coli, A2-K113) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse S100A9 amount of sample captured in plate.
Synonyms: Leukocyte L1 complex heavy chain|60B8AG|CAGB|Calgranulin B|Calgranulin-B|Calprotectin L1H subunit|CFAG|CGLB|Cystic fibrosis antigen|Cystic fibrosis antigen B|HGNC:10499|L1AG|Leukocyte L1 complex heavy chain|LIAG|MAC 387|MAC387|MIF|Migration inhibitory factor related protein 14|Migration inhibitory factor-related protein 14|MRP 14|MRP-14|MRP14|Myeloid-related protein 14|NIF|OTTHUMP00000015331|P14|Protein S100-A9|S100 A9|S100 calcium binding protein A9|S100 calcium binding protein A9 calgranulin B|S100 calcium-binding protein A9|S100A9|S10A9_HUMAN
Species: mouse S100A9
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse S100A9
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, A2-K113
Gene name: Protein S100-A9 / S100 calcium binding protein A9
Protein name: Protein S100-A9
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|104|235|1022|110|231|1104
Standard deviation|4.47|12.92|50.07|6.16|16.17|64.03
CV(%)|4.3%|5.5%|4.9%|5.6%|7%|5.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 104 | 102 | 87 | 93 | 96 | 6.87 | 7.1% |
| Sample 2 | 235 | 236 | 253 | 261 | 246 | 11.12 | 4.5% |
| Sample 3 | 1022 | 944 | 918 | 1057 | 985 | 56.39 | 5.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1152-CAP|96-well plate precoated with anti- mouse S100A9 antibody|1
EK1152-ST|Lyophilized recombinant mouse S100A9 standard|2ng/tube×2
EK1152-DA|Biotinylated anti- mouse S100A9 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
