Description
Boster’s mouse IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S115-S270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1 alpha amount of sample captured in plate.
Synonyms: Hematopoietin 1|IL 1 alpha|Il 1a|IL1|IL1 ALPHA|IL1A|IL1F1|Ilia|Interleukin 1 alpha|Interleukin 1 alpha precursor|Interleukin1 alpha|OTTHUMP00000203692|OTTMUSP00000016430|Preinterleukin 1|Pro interleukin 1|Pro interleukin 1 alpha|Prointerleukin 1|RP23-160G19.8
Species: Mouse IL-1 alpha
Assay: 4.7pg/ml-300pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant mouse IL-1 alpha
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: E.coli,S115-S270
Gene name: Interleukin-1 alpha / interleukin 1, alpha
Protein name: Interleukin-1 alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|15|63|151|16|60|155
Standard deviation|1|2.7|6.64|1.18|3.3|7.28
CV(%)|6.7%|4.3%|4.4%|7.4%|5.5%|4.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 15 | 16 | 16 | 13 | 15 | 1.22 | 8.1% |
| Sample 2 | 63 | 68 | 60 | 67 | 64 | 3.2 | 5% |
| Sample 3 | 151 | 139 | 139 | 146 | 143 | 5.06 | 3.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0391-CAP|96-well plate precoated with anti- mouse IL-1 alpha antibody|1
EK0391-ST|Lyophilized recombinant mouse IL-1 alpha standard|10ng/tubex2
EK0391-DA|Biotinylated anti- mouse IL-1 alpha antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
