Mouse GDNF PicoKine™ Fast ELISA Kit

Mouse GDNF PicoKine™ Fast ELISA Kit

Brand:
Boster Bio
Storage:

The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Mouse GDNF with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. GDNF tissue specificity: Expressed in both the central nervous system (CNS) and in non-CNS tissues. Expressed in a highly dynamic pattern in the anterior neuroectoderm during the early stages of neurogenesis between E7.5 and E10.5. Beginning at E10.5, expression begins in mesenchymal tissues of several organs including the digestive tract, kidney, testis, frontonasal mass, tooth primordium, tongue, mandible, whisker follicles, ear, eye, limb bud and in distinct regions of the brain. Also expressed in the heart, ileum, liver and muscle. .   

 

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SKU: FEK0935 Category:

Description

Boster’s mouse GDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for GDNF has been precoated onto 96-well plates. Standards(sf21, S78-I211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GDNF amount of sample captured in plate.

Synonyms: Glial cell line-derived neurotrophic factor;mGDNF;Astrocyte-derived trophic factor;ATF;Gdnf;

Species: Mouse GDNF

Assay: 31.2pg/ml-2000pg/ml

Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse GDNF

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: polyclonal antibody from goat|polyclonal antibody from goat

Anticoagulant: heparin or EDTA

Immunogen: Expression system for standard: sf21; Immunogen sequence: S78-I211

Gene name: Glial cell line-derived neurotrophic factor / Glial cell line-derived neurotrophic factor

Protein name: Glial cell line-derived neurotrophic factor

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 101 227 789 101 217 855
Standard deviation 7.57 9.3 57.53 7.67 9.11 66.69
CV(%) 7.5% 4.1% 7% 7.6% 4.2% 7.8%

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 101 101 104 97 100 2.48 2.4%
Sample 2 227 204 214 227 218 9.66 4.4%
Sample 3 789 928 792 891 850 60.93 7.1%

*number of samples for each test n=16.

10ng/tube

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