Description
Boster’s mouse CXCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL9 has been precoated onto 96-well plates. Standards(E.coli, T22-T126) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL9 amount of sample captured in plate.
Synonyms: C-X-C motif chemokine 9|_x000D_Chemokine (C-X-C motif) ligand 9|_x000D_CMK|_x000D__x000D__x000D_Crg 10|_x000D_CXCL9|_x000D_CXCL9_HUMAN|_x000D_Gamma interferon induced monokine|_x000D_Gamma-interferon-induced monokine|_x000D_HuMIG|_x000D_MIG|_x000D_Monokine induced by gamma interferon|_x000D_Monokine induced by interferon gamma|_x000D_Monokine induced by interferon-gamma|_x000D_SCYB9|_x000D_Small inducible cytokine B9|_x000D_Small inducible cytokine subfamily B member 9|_x000D_Small-inducible cytokine B9
Species: Mouse CXCL9
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CXCL9
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: E.coli, T22-T126
Gene name: C-X-C motif chemokine 9 / chemokine (C-X-C motif) ligand 9
Protein name: C-X-C motif chemokine 9
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|86|228|891|79|240|896
Standard deviation|4.9|13.45|38.31|4.74|17.28|43
CV(%)|5.7%|5.9%|4.3%|6%|7.2%|4.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 86 | 84 | 92 | 82 | 86 | 3.74 | 4.3% |
| Sample 2 | 228 | 207 | 217 | 212 | 216 | 7.77 | 3.5% |
| Sample 3 | 891 | 891 | 830 | 879 | 872 | 25.16 | 2.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0733-CAP|96-well plate precoated with anti- mouse CXCL9 antibody|1
EK0733-ST|Lyophilized recombinant mouse CXCL9 standard|10ng/tube×2
EK0733-DA|Biotinylated anti- mouse CXCL9 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
