Description
Boster’s mouse CTLA4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CTLA4 has been precoated onto 96-well plates. Standards(NSO, A37-F162) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CTLA4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CTLA4 amount of sample captured in plate.
Synonyms: Activation B7-1 antigen|_x000D_B7|_x000D_BB1|_x000D__x000D__x000D_CD80|_x000D_CD80_HUMAN|_x000D_CTLA-4 counter-receptor B7.1|_x000D_T-lymphocyte activation antigen CD80
Species: Mouse CTLA4
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CTLA4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, A37-F162
Gene name: Cytotoxic T-lymphocyte protein 4 / cytotoxic T-lymphocyte-associated protein 4
Protein name: Cytotoxic T-lymphocyte protein 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|312|1627|4692|332|1618|5074
Standard deviation|17.47|69.96|304.98|20.25|77.66|334.88
CV(%)|5.6%|4.3%|6.5%|6.1%|4.8%|6.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 312 | 290 | 303 | 293 | 299 | 8.67 | 2.8% |
| Sample 2 | 1627 | 1423 | 1427 | 1577 | 1513 | 90.25 | 5.9% |
| Sample 3 | 4692 | 4185 | 4538 | 4697 | 4528 | 208.09 | 4.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0717-CAP|96-well plate precoated with anti- mouse CTLA4 antibody|1
EK0717-ST|Lyophilized recombinant mouse CTLA4 standard|10ng/tube×2
EK0717-DA|Biotinylated anti- mouse CTLA4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
