Mouse CRP/C Reactive Protein/PTX1 PicoKine™ One Step ELISA Kit

Brand:
Boster Bio
Storage:

For the quantitation of Mouse Crp concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).   

 

Available on backorder

SKU: FEK0977 Category:

Description

The Boster One Step Picokine™ Mouse Crp Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Crp with a 96-well strip plate that is pre-coated with antibody specific for Crp. The detection antibody is a HRP conjugated antibody specific for Crp. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Crp with immunogen: Expression system for standard: NSO; Immunogen sequence: H20-S225. The kit is analytically validated with ready to use reagents.
To measure Mouse Crp, add standards and samples to the wells, then add HRP conjugated detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Crp in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse Crp in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster’s ELISA Resource Center at https://www.bosterbio.com/elisa-technical-resource-center.


Synonyms: Crp; |Ptx1; C-reactive protein

Species: Mouse Crp

Assay: 156pg/ml-10000pg/ml

Sensitivy: <10pg/ml Specifity: Natural and recombinant Mouse Crp

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: monoclonal antibody from rat|polyclonal antibody from goat

Anticoagulant: heparin, EDTA

Immunogen: Expression system for standard: NSO; Immunogen sequence: H20-S225

Gene name: C-reactive protein /

Protein name: C-reactive protein

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.(Shipped with wet ice.)


|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|254|1752|6770|265|1851|6930
Standard deviation|11.93|94.6|453.59|12.98|105.5|512.82
CV(%)|4.7%|5.4%|6.7%|4.9%|5.7%|7.4%


Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 254 243 280 249 256 13.82 5.4%
Sample 2 1752 1563 1416 1662 1598 97.47 6.1%
Sample 3 6770 5545 5924 6118 6089 395.78 6.5%

*number of samples for each test n=16.


10ng/tube