Description
Boster’s mouse CRLF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Monoclonal antibody from rat specific for CRLF2 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A20 – L233) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRLF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CRLF2 amount of sample captured in plate.
Synonyms: Cytokine receptor-like factor 2; Cytokine receptor-like molecule 2; CRLM-2; Thymic stromal lymphopoietin protein receptor; TSLP receptor; Type I cytokine receptor delta 1; Crlf2; Crlm2; Tpte2; Tslpr
Species: Mouse CRLF2
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CRLF2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,A20 – L233
Gene name: Cytokine receptor-like factor 2 / cytokine receptor-like factor 2
Protein name: Cytokine receptor-like factor 2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|155|962|6142|150|902|6620
Standard deviation|9.76|74.07|405.37|11.85|71.25|450.16
CV(%)|6.3%|7.7%|6.6%|7.9%|7.9%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 155 | 180 | 182 | 156 | 168 | 12.77 | 7.6% |
| Sample 2 | 962 | 986 | 889 | 968 | 951 | 37 | 3.8% |
| Sample 3 | 6142 | 5679 | 5442 | 6137 | 5850 | 301.38 | 5.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1665-CAP|96-well plate precoated with anti- mouse CRLF2 antibody|1
EK1665-ST|Lyophilized recombinant mouse CRLF2 standard|10ng/tube×2
EK1665-DA|Biotinylated anti- mouse CRLF2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
