Description
Boster’s mouse Clusterin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Clusterin has been precoated onto 96-well plates. Standards(NSO, D22-R226(β)&S227-E448(α)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Clusterin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Clusterin amount of sample captured in plate.
Synonyms: AAG4|Aging associated protein 4|APO J|APOJ|CLI|CLU|CLU1|CLU2|Clusterin|Complement associated protein SP 40,40|Complement cytolysis inhibitor|Complement lysis inhibitor|Ku70 binding protein 1|KUB1|NA1/NA2|SGP 2|SGP2|SP 40|SP40|Sulfated glycoprotein 2|Testosterone repressed prostate message 2|TRPM 2|TRPM2
Species: Mouse Clusterin
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse Clusterin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, D22-R226(beta)&S227-E448(alpha)
Gene name: Clusterin / clusterin
Protein name: Clusterin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|519|2684|8267|522|2616|7616
Standard deviation|30.1|112.72|537.35|36.54|122.95|632.12
CV(%)|5.8%|4.2%|6.5%|7%|4.7%|8.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 519 | 559 | 591 | 524 | 548 | 29.09 | 5.3% |
| Sample 2 | 2684 | 2395 | 2417 | 2667 | 2540 | 135.1 | 5.3% |
| Sample 3 | 8267 | 8908 | 7881 | 9107 | 8540 | 491.35 | 5.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0923-CAP|96-well plate precoated with anti- mouse Clusterin antibody|1
EK0923-ST|Lyophilized recombinant mouse Clusterin standard|20ng/tube×2
EK0923-DA|Biotinylated anti- mouse Clusterin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
