Description
Boster’s mouse CD30L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30L has been precoated onto 96-well plates. Standards(NSO, Q68-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30L amount of sample captured in plate.
Synonyms: CD153 antigen|_x000D_CD30 antigen ligand|_x000D_CD30 L|_x000D__x000D__x000D_CD30 ligand|_x000D_CD30L|_x000D_CD30LG|_x000D_MGC138144|_x000D_TNFSF8|_x000D_Tumor necrosis factor (ligand) superfamily member 8|_x000D_Tumor necrosis factor ligand superfamily member 8
Species: Mouse CD30L
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <6pg/ml Specifity: Natural and recombinant mouse CD30L
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q68-D239
Gene name: Tumor necrosis factor ligand superfamily member 8 / tumor necrosis factor (ligand) superfamily, member 8
Protein name: Tumor necrosis factor ligand superfamily member 8
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|35|254|445|38|267|477
Standard deviation|2.24|16.51|34.26|2.58|21.09|43.4
CV(%)|6.4%|6.5%|7.7%|6.8%|7.9%|9.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 30 | 34 | 31 | 32 | 2.06 | 6.4% |
| Sample 2 | 254 | 260 | 260 | 224 | 249 | 14.92 | 5.9% |
| Sample 3 | 445 | 507 | 470 | 490 | 478 | 23.11 | 4.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0572-CAP|96-well plate precoated with anti- mouse CD30L antibody|1
EK0572-ST|Lyophilized recombinant mouse CD30L standard|10ng/tubex2
EK0572-DA|Biotinylated anti- mouse CD30L antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
