Description
Boster’s mouse CCL12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL12 has been precoated onto 96-well plates. Standards(E.coli, G23-G104) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL12 amount of sample captured in plate.
Synonyms: C C motif chemokine 12|Ccl12|MCP 1 related chemokine|Monocyte chemoattractant protein 5|Monocyte chemotactic protein 5|Scya12|Small inducible cytokine A12
Species: mouse CCL12
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CCL12
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, G23-G104
Gene name: C-C motif chemokine 12 / chemokine (C-C motif) ligand 12
Protein name: C-C motif chemokine 12
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|88|249|906|82|260|979
Standard deviation|6.6|18.67|44.39|7.38|21.58|63.63
CV(%)|7.5%|7.5%|4.9%|9%|8.3%|6.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 88 | 91 | 79 | 83 | 85 | 4.6 | 5.4% |
| Sample 2 | 249 | 282 | 291 | 290 | 278 | 17.1 | 6.1% |
| Sample 3 | 906 | 880 | 837 | 800 | 855 | 40.53 | 4.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1128-CAP|96-well plate precoated with anti- mouse CCL12 antibody|1
EK1128-ST|Lyophilized recombinant mouse CCL12 standard|10ng/tube×2
EK1128-DA|Biotinylated anti- mouse CCL12 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
