Description
Boster’s mouse Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO, E18-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Adiponectin amount of sample captured in plate.
Synonyms: Adiponectin;30 kDa adipocyte complement-related protein;Adipocyte complement-related 30 kDa protein;ACRP30;Adipocyte, C1q and collagen domain-containing protein;Adipocyte-specific protein AdipoQ;Adipoq;Acdc, Acrp30, Apm1;
Species: Mouse Adiponectin
Assay: 312pg/ml-20,000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant full length mouse Adiponectin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from rat|polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: Expression system for standard: NSO; Immunogen sequence: E18-N244
Gene name: Adiponectin / Adiponectin
Protein name: Adiponectin
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 567 | 2994 | 7572 | 512 | 3121 | 7518 |
| Standard deviation | 37.98 | 179.64 | 318.02 | 37.75 | 240.31 | 330.79 |
| CV(%) | 6.7% | 6% | 4.2% | 7.1% | 7.7% | 4.4% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 567 | 603 | 628 | 571 | 592 | 24.91 | 4.2% |
| Sample 2 | 2994 | 2867 | 2845 | 2816 | 2880 | 67.97 | 2.3% |
| Sample 3 | 7572 | 7510 | 8167 | 7413 | 7665 | 295.03 | 3.8% |
*number of samples for each test n=16.
20ng/tube
