Description
Boster’s Monkey SDF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SDF-1 has been precoated onto 96-well plates. Standards(E.coli, K22-M93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SDF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey SDF-1 amount of sample captured in plate.
Synonyms: Stromal cell-derived factor 1 ;Cxcl12 ;
Species: Monkey SDF-1
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity:
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from rat|polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: Expression system for standard: E.coli; Immunogen sequence: K22-M93
Gene name: Stromal cell-derived factor 1 / chemokine (C-X-C motif) ligand 12
Protein name: Stromal cell-derived factor 1
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.67|1.6|2.8|0.91|2.1|3.2
Standard deviation|0.027|0.056|0.126|0.053|0.107|0.202
CV(%)|4|3.5|4.5|5.8|5.4|6.3
10ng/tube
