Description
Boster’s Monkey APP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for APP has been precoated onto 96-well plates. Standards(NSO, L18-L688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey APP amount of sample captured in plate.
Synonyms: Amyloid beta A4 protein;ABPP;APPI;APP;Alzheimer disease amyloid protein;Cerebral vascular amyloid peptide;CVAP;PreA4;Protease nexin-II;PN-II;N-APP;Soluble APP-alpha;S-APP-alpha;Soluble APP-beta;S-APP-beta;C99;Beta-amyloid protein 42;Beta-APP42;Beta-amyloid protein 40;Beta-APP40;C83;P3(42);P3(40);C80;Gamma-secretase C-terminal fragment 59;Amyloid intracellular domain 59;AICD-59;AID(59);Gamma-CTF(59);Gamma-secretase C-terminal fragment 57;Amyloid intracellular domain 57;AICD-57;AID(57);Gamma-CTF(57);Gamma-secretase C-terminal fragment 50;Amyloid intracellular domain 50;AICD-50;AID(50);Gamma-CTF(50);C31;APP;A4, AD1;
Species: Monkey APP
Assay: 312pg/ml-20,000pg/ml
Sensitivy: <100pg/ml Specifity:
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: polyclonal antibody from goat|polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: Expression system for standard: NSO; Immunogen sequence: L18-L688
Gene name: Amyloid beta A4 protein / Amyloid beta A4 protein
Protein name: Amyloid beta A4 protein
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(ng/ml) | 3.37 | 6.58 | 13.29 | 3.42 | 6.68 | 14.21 |
| Standard deviation | 0.162 | 0.336 | 0.704 | 0.198 | 0.414 | 0.895 |
| CV(%) | 4.8 | 6.3 | 5.3 | 5.8 | 6.2 | 6.3 |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 775 | 742 | 738 | 700 | 738 | 26.58 | 3.6% |
| Sample 2 | 2619 | 3033 | 2635 | 3053 | 2835 | 208.19 | 7.3% |
| Sample 3 | 9426 | 9494 | 8945 | 9504 | 9342 | 231.3 | 2.4% |
*number of samples for each test n=16.
20ng/tube
