A chemiluminescent probe; used to detect superoxide production by enzymatic and cellular sources; produces similar chemiluminescence in isolated aortic and cardiac tissues from wild-type and Nox1-Nox2-Nox4 triple knockout mice

Lucigenin – 100 mg

Brand:
Cayman
CAS:
2315-97-1
Storage:
-20
UN-No:
Non-Hazardous - /

Lucigenin is a chemiluminescent probe used to detect superoxide production and the presence of chloride.{24234,24236} It can be used to detect superoxide production by enzymatic and cellular sources.{24234,24232,7968} It is a sensitive method that has been applied to the monitoring of superoxide production from xanthine oxidase, microsomal NADPH cytochrome reductase, and NADPH oxidases of phagocytes, endothelial cells, fibroblasts, and smooth muscle cells of blood vessel walls.{24232,21946} However, it produces similar chemiluminescence signals in isolated aortic and cardiac tissues from wild-type and Nox1-Nox2-Nox4 triple knockout mice, suggesting that it is not selective for NADPH-based ROS production.{41846} It also reacts with hydrogen peroxide without generating free radical intermediates and has been used to detect lipid hydroperoxide in oils.{41847} Lucigenin is also used as a fluorescent chloride-sensitive indicator, with its fluorescence being quenched by chloride (ex/em = 455/505 nm, respectively).{24236,24235,24233} Lucigenin fluorescence is insensitive to phosphate, sulfate, and nitrate.{24236}  

 

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Description

A chemiluminescent probe; used to detect superoxide production by enzymatic and cellular sources; produces similar chemiluminescence in isolated aortic and cardiac tissues from wild-type and Nox1-Nox2-Nox4 triple knockout mice, suggesting that it is not selective for NADPH-based ROS production; reacts with hydrogen peroxide without generating free radical intermediates; a fluorescent chloride-sensitive indicator, with its fluorescence being quenched by chloride (ex/em = 455/505 nm, respectively)

Formal name: 10,10′-dimethyl-9,9′-biacridinium, dinitrate

Synonyms:  L-6,868|NSC 151912

Molecular weight: 510.5

CAS: 2315-97-1

Purity: ≥95%

Formulation: A crystalline solid

Application|Chemiluminescence||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Labeling & Detection|Reactive Probes||Research Area|Oxidative Stress & Reactive Species|Lipid Peroxidation||Research Area|Oxidative Stress & Reactive Species|Reactive Oxygen

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