An inhibitor of DDHD2 (IC50 = 1.3 nM); selective for DDHD2 over greater than 50 serine hydrolases

KLH45 – 10 mg

Brand:
Cayman
CAS:
1632236-44-2
Storage:
-20
UN-No:
Non-Hazardous - /

KLH45 is an inhibitor of the phospholipase DDHD domain containing 2 (DDHD2; IC50 = 1.3 nM).{43899} It is selective for DDHD2 over greater than 50 serine hydrolases, including DDHD1 and Sec23ip, but does inhibit α/β-hydrolase domain-containing protein 6 (ABHD6). It inhibits DDHD2 and ABHD6 in Neuro2A cells when used at a concentration of less than 10 nM and inhibits DDHD2 by greater than 95% at a concentration of 25 nM. KLH45 (2 µM) prevents increases in triacylglycerol (TAG) hydrolase activity in HEK293T cell lysates expressing recombinant DDHD2 and incubated with a radiolabeled TAG substrate. It decreases TAG levels in the CNS of mice when administered at a dose of 20 mg/kg twice per day for four days. KLH45 (2 µM) also reverses DDHD2-induced lipid droplet reduction in COS-7 cells expressing recombinant DDHD2 and loaded with oleic acid.{43900}  

 

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SKU: 19889 - Category:

Description

An inhibitor of DDHD2 (IC50 = 1.3 nM); selective for DDHD2 over greater than 50 serine hydrolases, including DDHD1 and Sec23ip, but does inhibit ABHD6; inhibits DDHD2 and ABHD6 in Neuro2A cells at 95% at 25 nM; prevents increases in TAG hydrolase activity in HEK293T cell lysates expressing DDHD2 and incubated with a radiolabeled TAG substrate at 2 µM; decreases TAG levels in mouse CNS at 20 mg/kg twice per day for four days; reverses DDHD2-induced lipid droplet reduction in COS-7 cells expressing species recombinant DDHD2 and loaded with oleic acid at 2 µM

Formal name: N-cyclohexyl-N-(2-phenylethyl)-4-[4-(trifluoromethoxy)phenyl]-2H-1,2,3-triazole-2-carboxamide

Synonyms: 

Molecular weight: 458.5

CAS: 1632236-44-2

Purity: ≥98%

Formulation: A solution in ethanol

Product Type|Biochemicals|Small Molecule Inhibitors|Glycerolipid Lipases||Product Type|Biochemicals|Small Molecule Inhibitors|Phospholipases||Research Area|Lipid Biochemistry|Glycerolipids||Research Area|Lipid Biochemistry|Glycerophospholipids|Phospholipases||Research Area|Neuroscience

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