A membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis; changes fluorescence characteristics with alteration in mitochondrial transmembrane potential

JC-1 – 1 mg

Brand:
Cayman
CAS:
3520-43-2
Storage:
-20
UN-No:
Non-Hazardous - /

JC-1 is a membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis.{14421,14323,14322} It selectively enters mitochondria and changes fluorescence characteristics with alteration in mitochondrial transmembrane potential (ΔΨm). In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as J-aggregates, which fluoresce red/orange and may be detected using FL2 settings by flow cytometry.{14322} A drop in ΔΨm, a very early event in apoptosis, results in JC-1 monomers, which fluoresce green (FL1 settings on flow cytometers).{14323,14322} JC-1 may also be used with fluorescent microscopes or plate readers, using excitation at 520-570 nm and emission at 570-610 nm for J-aggregates and excitation at 485 nm and emission at 535 nm for monomers.  

 

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Description

A membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis; changes fluorescence characteristics with alteration in mitochondrial transmembrane potential, a very early event in apoptosis

Formal name: 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-propen-1-yl]-1,3-diethyl-1H-benzimidazolium, monoiodide

Synonyms:  CBIC2|NK 1420

Molecular weight: 652.2

CAS: 3520-43-2

Purity: ≥90%

Formulation: A crystalline solid

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Mitochondrial Biology

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