Description
Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.{53880,53881,53882} It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in rabbit serum. IgG consists of two identical heavy chains of approximately 50 kDa each and two identical light chains of approximately 25 kDa each.{53880,53881} The heavy chains are linked together by a single disulfide bond to form an Fc region and also combine with the light chains through additional disulfide bonds to form the Fab region, which mediate receptor and antigen binding, respectively. IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.{53880,53881,55170,53883} Rabbits express three light chains, IgGκ (K1), IgGκ (K2), and IgGγ, with IgGκ (K1)-containing IgGs comprising approximately 90% of the total IgG population. Unlike human and mouse IgG, rabbit IgG has a single subclass and a short upper and middle hinge length of only 11 residues that facilitates the hinge extension necessary for binding of the Fc receptor (FcR) and activating complement component 1q (C1q) to facilitate complement activation.{53880,53882} Cayman’s IgG Fab (rabbit) Monoclonal Antibody can be used for ELISA and Western blot (WB) applications. The antibody recognizes the Fab region of non-reduced IgG at approximately 150 kDa from rabbit samples.
Synonyms: Immunoglobulin G
Immunogen: Rabbit IgG
Formulation: 100 µg of protein G-affinity purified monoclonal antibody
Isotype: IgG
Applications: ELISA, WB (non-reducing conditions)
Origin:
Stability: 365 days
Application|ELISA||Application|Flow Cytometry||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies||Research Area|Cardiovascular System|Blood|Serum Proteins||Research Area|Immunology & Inflammation|Adaptive Immunity||Research Area|Immunology & Inflammation|Innate Immunity||Research Area|Infectious Disease
