Description
Boster’s human TRAILR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRAILR3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A26-A236) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRAILR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TRAILR3 amount of sample captured in plate.
Synonyms: Tumor necrosis factor receptor superfamily member 10C|Antagonist decoy receptor for TRAIL/Apo-2L|Decoy TRAIL receptor without death domain|Decoy receptor 1|DcR1|Lymphocyte inhibitor of TRAIL|TNF-related apoptosis-inducing ligand receptor 3|TRAIL receptor 3|TRAIL-R3|TRAIL receptor without an intracellular domain|CD263|TNFRSF10C|DCR1|LIT|TRAILR3|TRID
Species: Human TNFRSF10C
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <12pg/ml Specifity: Natural and recombinant human TRAILR3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, A26-A236
Gene name: Tumor necrosis factor receptor superfamily member 10C / TNF receptor superfamily member 10c
Protein name: Tumor necrosis factor receptor superfamily member 10C
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|154|584|1628|145|568|1473
Standard deviation|8.31|40.88|109.07|9.42|46|103.11
CV(%)|5.4%|7%|6.7%|6.5%|8.1%|7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 154 | 163 | 147 | 142 | 151 | 7.88 | 5.2% |
| Sample 2 | 584 | 548 | 550 | 549 | 557 | 15.17 | 2.7% |
| Sample 3 | 1628 | 1534 | 1759 | 1601 | 1630 | 81.7 | 5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity_x000D_
EK0533-CAP|96-well plate precoated with anti- human TRAILR3 antibody|1_x000D_
EK0533-ST|Lyophilized recombinant human TRAILR3 standard|10ng/tube×2_x000D_
EK0533-DA|Biotinylated anti- human TRAILR3 antibody|130μl(dilution 1:100)_x000D_
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)_x000D_
AR1106-1|Sample diluent buffer|30ml_x000D_
AR1106-2|Antibody diluent buffer|12ml_x000D_
AR1106-3|ABC diluent buffer|12ml_x000D_
AR1104|TMB color developing agent|10ml_x000D_
AR1105|TMB stop solution|10ml_x000D_
PLA-SEA|Adhesive cover|4
