Description
Boster’s human TNF beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNF beta has been precoated onto 96-well plates. Standards(E.coli, L35-L205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNF beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNF beta amount of sample captured in plate.
Synonyms: Coley’s toxin|_x000D_DAMA-25N12.13-004|_x000D_DIF|_x000D__x000D__x000D_Differentiation inducing factor|_x000D_Hemorrhagic factor |_x000D_LT|_x000D_LT alpha|_x000D_LT-alpha|_x000D_Lta|_x000D_LTalpha|_x000D_Lymphotoxin alpha|_x000D_Lymphotoxin alpha (TNF superfamily, member 1)|_x000D_Lymphotoxin-alpha|_x000D_MGC117668|_x000D_Natural killer cytotoxic factor|_x000D_Necrosin|_x000D_NKCF|_x000D_OTTHUMP00000037612|_x000D_OTTHUMP00000037613|_x000D_TNF B|_x000D_TNF superfamily member 1|_x000D_TNF-beta|_x000D_TNFB|_x000D_TNFB_HUMAN|_x000D_TNFbeta|_x000D_TNFSF 1|_x000D_TNFSF1|_x000D_Tumor necrosis factor beta|_x000D_Tumor necrosis factor ligand superfamily member 1|_x000D_Tumor necrosis factor superfamily member 1
Species: Human TNF beta
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human TNF beta
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, L35-L205
Gene name: Lymphotoxin-alpha / lymphotoxin alpha
Protein name: Lymphotoxin-alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|165|487|1791|179|521|1896
Standard deviation|11.71|32.14|127.16|13.06|38.55|157.36
CV(%)|7.1%|6.6%|7.1%|7.3%|7.4%|8.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 165 | 162 | 154 | 142 | 155 | 8.89 | 5.7% |
| Sample 2 | 487 | 454 | 458 | 413 | 453 | 26.37 | 5.8% |
| Sample 3 | 1791 | 1778 | 1760 | 1730 | 1764 | 22.88 | 1.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0584-CAP|96-well plate precoated with anti- human TNF beta antibody|1
EK0584-ST|Lyophilized recombinant human TNF beta standard|10ng/tubex2
EK0584-DA|Biotinylated anti- human TNF beta antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
