Description
Boster’s human TNF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNF alpha has been precoated onto 96-well plates. Standards (E.coli,V77-L233) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNF alpha amount of sample captured in plate.
Synonyms: APC1 protein|_x000D_Cachectin|_x000D_DIF|_x000D__x000D__x000D_soluble form|_x000D_Tnf|_x000D_TNF a|_x000D_TNF alpha|_x000D_TNF macrophage derived|_x000D_TNF monocyte derived|_x000D_TNF superfamily member 2|_x000D_TNF-a|_x000D_TNF-alpha|_x000D_TNFA_HUMAN|_x000D_TNFSF2|_x000D_Tumor necrosis factor|_x000D_Tumor necrosis factor alpha|_x000D_Tumor necrosis factor ligand superfamily member 2
Species: Human TNF alpha
Assay: 15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(serum,plasma)
Sensitivy: <1pg/ml Specifity: Natural and recombinant human TNF alpha
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli,V77-L233
Gene name: Tumor necrosis factor / tumor necrosis factor
Protein name: Tumor necrosis factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|16|82|201|14|75|186
Standard deviation|0.76|5.49|12.66|0.75|5.55|11.9
CV(%)|4.8%|6.7%|6.3%|5.4%|7.4%|6.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 15 | 15 | 16 | 15 | 0.5 | 3.3% |
| Sample 2 | 82 | 91 | 75 | 89 | 84 | 6.29 | 7.4% |
| Sample 3 | 201 | 204 | 215 | 219 | 209 | 7.46 | 3.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0525-CAP|96-well plate precoated with anti- human TNF alpha antibody|1
EK0525-ST|Lyophilized recombinant human TNF alpha standard|10ng/tubex2
EK0525-DA|Biotinylated anti- human TNF alpha antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
