Description
Boster’s human TGFBR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFBR3 has been precoated onto 96-well plates. Standards(NSO,G21-D781) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFBR3 amount of sample captured in plate.
Synonyms: Beta glycan|Betaglycan|Betaglycan proteoglycan|BGCAN|TGBR3_HUMAN|TGF beta receptor type 3|TGF beta receptor type III|TGF-beta receptor type 3|TGF-beta receptor type III|TGFB R3|TGFBR 3|TGFBR3|TGFR 3|TGFR-3|TGFR3|Transforming growth factor beta receptor III|
Transforming growth factor beta receptor III betaglycan 300kDa|Transforming growth factor beta receptor type 3
Species: human TGFBR3
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human TGFBR3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, G21-D781
Gene name: Transforming growth factor beta receptor type 3 / transforming growth factor, beta receptor III
Protein name: Transforming growth factor beta receptor type 3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|186|1370|5554|182|1499|5882
Standard deviation|8.74|102.75|294.36|11.83|133.41|376.44
CV(%)|4.7%|7.5%|5.3%|6.5%|8.9%|6.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 186 | 199 | 223 | 196 | 201 | 13.58 | 6.7% |
| Sample 2 | 1370 | 1157 | 1296 | 1222 | 1261 | 79.75 | 6.3% |
| Sample 3 | 5554 | 6224 | 5744 | 5545 | 5766 | 275.69 | 4.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1383-CAP|96-well plate precoated with anti- human TGFBR3 antibody|1
EK1383-ST|Lyophilized recombinant human TGFBR3 standard|10ng/tube×2
EK1383-DA|Biotinylated anti- human TGFBR3 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
