Description
Boster’s human TGF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF alpha has been precoated onto 96-well plates. Standards (E.coli,V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGF alpha amount of sample captured in plate.
Synonyms: EGF like TGF|_x000D_ETGF|_x000D_OTTHUMP00000203300|_x000D__x000D__x000D_OTTHUMP00000203301|_x000D_OTTHUMP00000203345|_x000D_OTTHUMP00000203352|_x000D_Protransforming growth factor alpha|_x000D_RATTGFAA|_x000D_TFGA|_x000D_TGF A|_x000D_TGF type 1|_x000D_TGFA|_x000D_Transforming growth factor alpha|_x000D_Transforming growth factor alpha precursor|_x000D_Wa1|_x000D_Waved 1
Species: Human TGF alpha
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human TGF alpha
Cross Reactivity: There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1%. Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli,V40-A89
Gene name: Protransforming growth factor alpha / transforming growth factor, alpha
Protein name: Protransforming growth factor alpha
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|31|230|448|29|237|431
Standard deviation|1.98|16.56|34.49|2.4|18.96|34.04
CV(%)|6.4%|7.2%|7.7%|8.3%|8%|7.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 33 | 31 | 35 | 32 | 1.65 | 5.1% |
| Sample 2 | 230 | 219 | 227 | 246 | 230 | 9.81 | 4.2% |
| Sample 3 | 448 | 498 | 470 | 454 | 467 | 19.35 | 4.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0511-CAP|96-well plate precoated with anti- human TGF alpha antibody|1
EK0511-ST|Lyophilized recombinant human TGF alpha standard|10ng/tubex2
EK0511-DA|Biotinylated anti- human TGF alpha antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
