Description
Boster’s human Survivin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Survivin has been precoated onto 96-well plates. Standards(E.coli, M1-D142) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Survivin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Survivin amount of sample captured in plate.
Synonyms: API4|_x000D_Apoptosis inhibitor 4|_x000D_Apoptosis inhibitor survivin|_x000D__x000D__x000D_Baculoviral IAP repeat containing 5|_x000D_Baculoviral IAP repeat-containing protein 5|_x000D_BIRC5|_x000D_BIRC5_HUMAN|_x000D_EPR 1
Species: Human Survivin
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human Survivin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, M1-D142
Gene name: Baculoviral IAP repeat-containing protein 5 / baculoviral IAP repeat containing 5
Protein name: Baculoviral IAP repeat-containing protein 5
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|207|660|1259|192|690|1195
Standard deviation|15.52|48.18|51.61|15.93|57.27|66.92
CV(%)|7.5%|7.3%|4.1%|8.3%|8.3%|5.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 207 | 178 | 185 | 184 | 188 | 11.01 | 5.8% |
| Sample 2 | 660 | 723 | 712 | 743 | 709 | 30.66 | 4.3% |
| Sample 3 | 1259 | 1187 | 1079 | 1117 | 1160 | 68.8 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0641-CAP|96-well plate precoated with anti- human Survivin antibody|1
EK0641-ST|Lyophilized recombinant human Survivin standard|4ng/tubex2
EK0641-DA|Biotinylated anti- human Survivin antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
