Description
Boster’s human sTNFsRⅡ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sTNFsRⅡ has been precoated onto 96-well plates. Standards(NSO, L23-D257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sTNFsRⅡ is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sTNFsRⅡ amount of sample captured in plate.
Synonyms: CD120b|Etanercept|p75|p80 TNF alpha receptor|TBP2|TBPII|TNF R2|TNFBR|TNFR-II|TNFR2|TNFRII|Tumor necrosis factor binding protein 2|Tumor necrosis factor receptor 2|Tumor necrosis factor receptor superfamily member 1B|Tumor necrosis factor receptor superfamily member 1b, membrane form|Tumor necrosis factor receptor type II
Species: Human sTNFsRⅡ
Assay: 7.8pg/ml-500pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human sTNFsRⅡ
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, L23-D257
Gene name: Tumor necrosis factor receptor superfamily member 1B / tumor necrosis factor receptor superfamily, member 1B
Protein name: Tumor necrosis factor receptor superfamily member 1B
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|16|58|235|14|55|233
Standard deviation|1|2.43|15.04|0.89|3.19|15.37
CV(%)|6.3%|4.2%|6.4%|6.4%|5.8%|6.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 16 | 17 | 16 | 16 | 0.43 | 2.6% |
| Sample 2 | 58 | 61 | 58 | 56 | 58 | 1.78 | 3% |
| Sample 3 | 235 | 274 | 227 | 275 | 252 | 21.93 | 8.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0530-CAP|96-well plate precoated with anti- human sTNFR II antibody|1
EK0530-ST|Lyophilized recombinant human sTNFR II standard|10ng/tubex2
EK0530-DA|Biotinylated anti- human sTNFR II antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
