Description
Boster’s human SMOC2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SMOC2 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: Q22-G446) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SMOC2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SMOC2 amount of sample captured in plate.
Synonyms: SPARC-related modular calcium-binding protein 2; Secreted modular calcium-binding protein 2; SMOC-2; Smooth muscle-associated protein 2; SMAP-2; SMOC2; SMAP2; MSTP117
Species: Human SMOC2
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human SMOC2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: CHO,Q22-G446
Gene name: SPARC-related modular calcium-binding protein 2 / SPARC related modular calcium binding 2
Protein name: SPARC-related modular calcium-binding protein 2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|142|785|1622|131|825|1562
Standard deviation|8.09|51.02|128.13|8.38|56.92|148.39
CV(%)|5.7%|6.5%|7.9%|6.4%|6.9%|9.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 142 | 143 | 136 | 132 | 138 | 4.49 | 3.2% |
| Sample 2 | 785 | 719 | 731 | 758 | 748 | 25.48 | 3.4% |
| Sample 3 | 1622 | 1861 | 1845 | 1879 | 1801 | 104.47 | 5.8% |
*number of samples for each test n=16.
Catalog number Description Quantity
EK1664-CAP 96-well plate precoated with anti- human SMOC2 antibody 1
EK1664-ST Lyophilized recombinant human SMOC2 standard 10ng/tube×2
EK1664-DA Biotinylated anti- human SMOC2 antibody 130μl(dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
AR1106-1 Sample diluent buffer 30 ml
AR1106-2 Antibody diluent buffer 12ml
AR1106-3 ABC diluent buffer 12ml
AR1104 TMB color developing agent 10ml
AR1105 TMB stop solution 10ml
PLA-SEA Adhesive cover 4
