Description
Boster’s human SHBG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SHBG has been precoated onto 96-well plates. Standards(NSO, L30-H402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SHBG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SHBG amount of sample captured in plate.
Synonyms: ABP|Androgen binding protein|SBP|Sex hormone-binding globulin|Sex steroid binding protein|Sex steroid-binding protein|SHBG|SHBG_ HUMAN|TeBG|Testis specific androgen binding protein|Testis-specific androgen-binding protein|Testosterone binding beta globulin| Testosterone estradiol binding globulin|Testosterone estrogen binding globulin|Testosterone-estradiol-binding globulin|Testosterone-estrogen-binding globulin
Species: human SHBG
Assay: 0.031nmol/L-2nmol/L
Sensitivy: <0.001nmol/L Specifity: Natural and recombinant human SHBG
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,L30-H402
Gene name: Sex hormone-binding globulin / sex hormone-binding globulin
Protein name: Sex hormone-binding globulin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|60|301|945|61|312|954
Standard deviation|3|18.66|60.48|3.66|22.46|67.73
CV(%)|5%|6.2%|6.4%|6%|7.2%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pmol/ml) | Lot2 (pmol/ml) | Lot3 (pmol/ml) | Lot4 (pmol/ml) | Mean (pmol/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 62 | 64 | 64 | 62 | 1.65 | 2.6% |
| Sample 2 | 301 | 260 | 295 | 294 | 287 | 16.1 | 5.6% |
| Sample 3 | 945 | 910 | 861 | 916 | 908 | 30.19 | 3.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1236-CAP|96-well plate precoated with anti- human SHBG antibody|1
EK1236-ST|Lyophilized recombinant human SHBG standard|0.002nmol/tube×2
EK1236-DA|Biotinylated anti- human SHBG antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
