Description
Boster’s human Serpin A5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Serpin A5 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E26-P406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin A5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Serpin A5 amount of sample captured in plate.
Synonyms: Plasma serine protease inhibitor; Acrosomal serine protease inhibitor; Plasminogen activator inhibitor 3; PAI-3; PAI3; Protein C inhibitor; PCI; Serpin A5; SERPINA5; PCI; PLANH3; PROCI
Species: Human Serpin A5/PAI3
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Serpin A5
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,E26-P406
Gene name: Plasma serine protease inhibitor / serine (or cysteine) peptidase inhibitor, clade A, member 5
Protein name: Plasma serine protease inhibitor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|225|1716|4489|220|1814|4577
Standard deviation|17.77|125.26|314.23|20.9|137.86|407.35
CV(%)|7.9%|7.3%|7%|9.5%|7.6%|8.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 225 | 227 | 232 | 228 | 228 | 2.54 | 1.1% |
| Sample 2 | 1716 | 1679 | 1599 | 1655 | 1662 | 42.49 | 2.5% |
| Sample 3 | 4489 | 3979 | 4488 | 4123 | 4269 | 224.59 | 5.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity_x000D_
EK1631-CAP|96-well plate precoated with anti- human Serpin A5 antibody|1
EK1631-ST|Lyophilized recombinant human Serpin A5 standard|10ng/tube×2
EK1631-DA|Biotinylated anti- human Serpin A5 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)_x000D_
AR1106-1|Sample diluent buffer|30ml_x000D_
AR1106-2|Antibody diluent buffer|12ml_x000D_
AR1106-3|ABC diluent buffer|12ml_x000D_
AR1104|TMB color developing agent|10ml_x000D_
AR1105|TMB stop solution|10ml_x000D_
PLA-SEA|Adhesive cover|4
