Description
Boster’s human Serpin A12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin A12 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L21-K414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12
Species: Human Serpin A12
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human Serpin A12
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, L21-K414
Gene name: Serpin A12 / serpin family A member 12
Protein name: Serpin A12
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|212|1109|5465|232|1235|6154
Standard deviation|11.24|68.76|245.93|10.90|79.04|332.32
CV(%)|5.3%|6.2%|4.5%|4.7%|6.4%|5.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 212 | 215 | 232 | 220 | 219 | 7.62 | 3.4% |
| Sample 2 | 1109 | 1215 | 1235 | 1149 | 1177 | 50.53 | 4.2% |
| Sample 3 | 5465 | 6356 | 5987 | 5976 | 5946 | 317.03 | 5.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1874-CAP|96-well plate precoated with anti- human Serpin A12 antibody|1
EK1874-ST|Lyophilized recombinant human Serpin A12 standard|10ng/tube×2
EK1874-DA|Biotinylated anti- human Serpin A12 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
