Description
Boster’s human SCGB1A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCGB1A1 has been precoated onto 96-well plates. Standards(E.coli, E22-N91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB1A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SCGB1A1 amount of sample captured in plate.
Synonyms: blastokinin|CC10|CC16|CCPBP|CCSP|Clara cell phospholipid binding protein|Clara cell phospholipid-binding protein|clara cell secretory protein|Clara cell specific 10 kD protein|Clara Cells 10 kDa Secretory Protein|OTTHUMP00000236107|SCGB1A1|Secretoglobin family 1A member 1|Secretoglobin, family 1A, member 1 (uteroglobin)|UG|UGB|UP-1|UP1|Urinary Protein 1|Urine Protein 1|UTER_HUMAN|Uteroglobin|Utg
Species: Human SCGB1A1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human SCGB1A1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, E22-N91
Gene name: Uteroglobin / secretoglobin, family 1A, member 1 (uteroglobin)
Protein name: Uteroglobin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|330|1779|5273|358|1704|5725
Standard deviation|26.07|124.53|406.02|28.99|119.28|538.15
CV(%)|7.9%|7%|7.7%|8.1%|7%|9.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 330 | 331 | 310 | 315 | 321 | 9.17 | 2.8% |
| Sample 2 | 1779 | 1757 | 1732 | 2013 | 1820 | 112.51 | 6.1% |
| Sample 3 | 5273 | 5090 | 5978 | 5483 | 5456 | 331.91 | 6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0922-CAP|96-well plate precoated with anti- human SCGB1A1 antibody|1
EK0922-ST|Lyophilized recombinant human SCGB1A1 standard|10ng/tube×2
EK0922-DA|Biotinylated anti- human SCGB1A1antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
