Description
Boster’s human S100A8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for S100A8 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: L2-E93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human S100A8 amount of sample captured in plate.
Synonyms: CAGA | CAGA | CFAG | MRP8 | Caga | Mrp8 | Calgranulin-A | Calprotectin L1L subunit | CFAG | Cystic fibrosis antigen | Leukocyte L1 complex light chain | MRP 8 | MRP-8 | P05109 | Protein S100A8 | Protein S100-A8 | S100 calcium binding protein A8 | S100 calcium-binding protein A8 | S10A8 | Urinary stone protein band A
Species: human S100A8
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human S100A8
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli,L2-E93
Gene name: Protein S100-A8 / S100 calcium binding protein A8
Protein name: Protein S100-A8
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|78|221|906|80|240|836
Standard deviation|4.75|9.28|36.24|5.68|10.08|43.47
CV(%)|6.1%|4.2%|4%|7.1%|4.2%|5.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 78 | 74 | 79 | 69 | 75 | 3.93 | 5.2% |
| Sample 2 | 221 | 264 | 241 | 238 | 241 | 15.31 | 6.3% |
| Sample 3 | 906 | 902 | 918 | 1013 | 934 | 45.55 | 4.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1558-CAP|96-well plate precoated with anti- human S100A8 antibody|1
EK1558-ST|Lyophilized recombinant human S100A8 standard|2ng/tube×2
EK1558-DA|Biotinylated anti- human S100A8 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
