Description
The Boster One Step Picokine™ Human PTX3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PTX3 with a 96-well strip plate that is pre-coated with antibody specific for PTX3. The detection antibody is a HRP conjugated antibody specific for PTX3. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human PTX3 with immunogen: Expression system for standard: NSO; Immunogen sequence: E18-S381. The kit is analytically validated with ready to use reagents.
To measure Human PTX3, add standards and samples to the wells, then add HRP conjugated detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human PTX3 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human PTX3 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster’s ELISA Resource Center at https://www.bosterbio.com/elisa-technical-resource-center.
Synonyms: PTX3; TNFAIP5; TSG14; Pentraxin-related protein PTX3; Pentaxin-related protein PTX3; Tumor necrosis factor alpha-induced protein 5; TNF alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; TSG-14
Species: Human PTX3
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant Human PTX3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: Expression system for standard: NSO; Immunogen sequence: E18-S381
Gene name: Pentraxin-related protein PTX3 / pentraxin 3
Protein name: Pentraxin-related protein PTX3
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|687|1807|9209|814|1923|9534
Standard deviation|37.09|110.22|635.42|56.16|138.45|762.72
CV(%)|5.4%|6.1%|6.9|6.9%|7.2%|8.0%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 687 | 618 | 703 | 627 | 658 | 36.19 | 5.5% |
| Sample 2 | 1807 | 1579 | 1636 | 1784 | 1701 | 81.64 | 4.8% |
| Sample 3 | 9209 | 8917 | 8805 | 8754 | 8921 | 330.07 | 3.7% |
*number of samples for each test n=16.
20ng/tube
