Description
Boster’s human PTX3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for PTX3 has been precoated onto 96-well plates. Standards(NSO, E18-S381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PTX3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PTX3 amount of sample captured in plate.
Synonyms: AI607804|Pentaxin 3|Pentaxin-related protein PTX3|Pentraxin 3 long|Pentraxin-related gene|Pentraxin-related gene rapidly induced by IL-1 beta|Pentraxin-related protein PTX3|PTX3|PTX3_HUMAN|TNF alpha-induced protein 5|TNFAIP5|TSG-14|TSG14|Tumor necrosis factor alpha-induced protein 5|Tumor necrosis factor-inducible gene 14 protein|Tumor necrosis factor-inducible protein TSG-14
Species: Human PTX3
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human PTX3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: NSO, E18-S381
Gene name: Pentraxin-related protein PTX3 / pentraxin 3, long
Protein name: Pentraxin-related protein PTX3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|662|1701|9194|718|1816|9601
Standard deviation|33.76|85.05|579.22|38.77|92.61|662.46
CV(%)|5.1%|5%|6.3%|5.4%|5.1%|6.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 662 | 620 | 693 | 604 | 644 | 34.99 | 5.4% |
| Sample 2 | 1701 | 1556 | 1536 | 1612 | 1601 | 63.97 | 3.9% |
| Sample 3 | 9194 | 8999 | 8940 | 8763 | 8974 | 153.86 | 1.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0861-CAP|96-well plate precoated with anti- human PTX3 antibody|1
EK0861-ST|Lyophilized recombinant human PTX3 standard|20ng/tube×2
EK0861-DA|Biotinylated anti- human PTX3 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
