Description
Boster’s human PSP94 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PSP94 has been precoated onto 96-well plates. Standards(E.coli, S21-I114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PSP94 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PSP94 amount of sample captured in plate.
Synonyms: Beta microseminoprotein [Precursor]|Beta-microseminoprotein|HPC 13|HPC13|IGBF|Immunoglobulin binding factor|Immunoglobulin-binding factor|microseminoprotein beta|Msmb|MSMB_HUMAN|MSP|MSPB|PN 44|PN44|Prostate secreted seminal plasma protein|Prostate secretory protein of 94 amino acids|Prostate secretory protein PSP94|PRPS|PRSP|PSP 57|PSP|PSP-94|PSP57|PSP94|Seminal plasma beta inhibin|Seminal plasma beta-inhibin
Species: human PSP94
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human PSP94
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, S21-I114
Gene name: Beta-microseminoprotein / microseminoprotein, beta-
Protein name: Beta-microseminoprotein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|160|628|1438|148|589|1345
Standard deviation|9.44|49.61|113.6|8.73|53.59|130.46
CV(%)|5.9%|7.9%|7.9%|5.9%|9.1%|9.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 160 | 156 | 160 | 151 | 156 | 3.69 | 2.3% |
| Sample 2 | 628 | 662 | 671 | 720 | 670 | 32.89 | 4.9% |
| Sample 3 | 1438 | 1274 | 1320 | 1318 | 1337 | 60.86 | 4.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1207-CAP|96-well plate precoated with anti- human PSP94 antibody|1
EK1207-ST|Lyophilized recombinant human PSP94 standard|10ng/tube×2
EK1207-DA|Biotinylated anti- human PSP94 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
