Description
Boster’s human PSG1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PSG1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q35-P419) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PSG1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Pregnancy-specific beta-1-glycoprotein 1; PS-beta-G-1; PSBG-1; Pregnancy-specific glycoprotein 1; CD66 antigen-like family member F; Fetal liver non-specific cross-reactive antigen 1/2; FL-NCA-1/2; PSG95; Pregnancy-specific beta-1 glycoprotein C/D; PS-beta-C/D; CD66f; PSG1; B1G1; PSBG; PSGGA
Species: Human PSG1
Assay: 1.56ng/ml-100ng/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human PSG1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q35-P419
Gene name: Pregnancy-specific beta-1-glycoprotein 1 / pregnancy specific beta-1-glycoprotein 1
Protein name: Pregnancy-specific beta-1-glycoprotein 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|2327|9714|48035|2915|11475|48927
Standard deviation|114.02|456.56|1969.44|163.24|585.23|3229.18
CV(%)|4.9%|4.7%|4.1%|5.6%|5.1%|6.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 2327 | 2279 | 2306 | 2344 | 2314 | 24.27 | 1.0% |
| Sample 2 | 9714 | 10138 | 10569 | 10990 | 10352 | 476.17 | 4.5% |
| Sample 3 | 48035 | 41237 | 47332 | 49070 | 46418 | 3054.74 | 6.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1997-CAP|96-well plate precoated with anti- human PSG1 antibody|1
EK1997-ST|Lyophilized recombinant human PSG1 standard|100ng/tube×2
EK1997-DA|Biotinylated anti- human PSG1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
