Description
Boster’s human P53 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P53 has been precoated onto 96-well plates. Standards(NSO, M1-D393) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P53 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P53 amount of sample captured in plate.
Synonyms: Antigen NY-CO-13|BCC7|Cellular tumor antigen p53|LFS1|MS980|p53|p53 tumor suppressor|P53_HUMAN|Phosphoprotein p53|Tp53|Transformation related protein 53|TRP53|Tumor suppressor p53
Species: Human P53
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human P53
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, M1-D393
Gene name: Cellular tumor antigen p53 / tumor protein p53
Protein name: Cellular tumor antigen p53
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|275|1540|5055|302|1396|5437
Standard deviation|16.5|72.38|394.29|20.83|71.19|467.58
CV(%)|6%|4.7%|7.8%|6.9%|5.1%|8.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 275 | 249 | 256 | 233 | 253 | 15.07 | 5.9% |
| Sample 2 | 1540 | 1645 | 1702 | 1738 | 1656 | 74.86 | 4.5% |
| Sample 3 | 5055 | 4442 | 4533 | 4238 | 4567 | 301.31 | 6.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0895-CAP|96-well plate precoated with anti- human P53 antibody|1
EK0895-ST|Lyophilized recombinant human P53 standard|10ng/tube×2
EK0895-DA|Biotinylated anti- human P53 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
