Description
Boster’s human P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P-Selectin has been precoated onto 96-well plates. Standards(NSO, W42-A771) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P-Selectin amount of sample captured in plate.
Synonyms: Antigen CD62|_x000D_CD62|_x000D_CD62 antigen like family member P|_x000D__x000D__x000D_CD62 antigen-like family member P|_x000D_CD62P|_x000D_FLJ45155|_x000D_GMP 140|_x000D_GMP-140|_x000D_GMP140|_x000D_Granule membrane protein 140|_x000D_Granule membrane protein 140kDa|_x000D_Granulocyte membrane protein|_x000D_GRMP|_x000D_LECAM3|_x000D_Leukocyte endothelial cell adhesion molecule 3|_x000D_Leukocyte-endothelial cell adhesion molecule 3|_x000D_LYAM3_HUMAN|_x000D_P selectin|_x000D_P-selectin|_x000D_PADGEM|_x000D_Platelet activation dependent granule external membrane protein|_x000D_Platelet activation dependent granule-external membrane protein|_x000D_Platelet alpha granule membrane protein|_x000D_PSEL|_x000D_Selectin P|_x000D_Selectin P (granule membrane protein 140kDa antigen CD62)|_x000D_SELP
Species: Human P-Selectin
Assay: 156pg/ml-10000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human sP-Selectin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, W42-A771
Gene name: P-selectin / selectin P (granule membrane protein 140kDa, antigen CD62)
Protein name: P-selectin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|322|1820|5020|331|1849|5389
Standard deviation|15.13|121.94|256.02|21.18|144.22|301.78
CV(%)|4.7%|6.7%|5.1%|6.4%|7.8%|5.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 322 | 316 | 323 | 357 | 329 | 16.1 | 4.8% |
| Sample 2 | 1820 | 1756 | 2051 | 1775 | 1850 | 118.06 | 6.3% |
| Sample 3 | 5020 | 5825 | 5171 | 5716 | 5433 | 343.86 | 6.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0505-CAP|96-well plate precoated with anti- human sP-Selectin antibody|1
EK0505-ST|Lyophilized recombinant human sP-Selectin standard|10ng/tubex2
EK0505-DA|Biotinylated anti- human sP-Selectin antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
