Description
Boster’s human P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P-Selectin has been precoated onto 96-well plates. Standards(NSO, W42-A771) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P-Selectin amount of sample captured in plate.
Synonyms: P-selectin;CD62 antigen-like family member P;Granule membrane protein 140;GMP-140;Leukocyte-endothelial cell adhesion molecule 3;LECAM3;Platelet activation dependent granule-external membrane protein;PADGEM;CD62P;SELP;GMRP, GRMP;
Species: Human P-Selectin
Assay: 156pg/ml-10000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human sP-Selectin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: Expression system for standard: NSO; Immunogen sequence: W42-A771
Gene name: P-selectin / P-selectin
Protein name: P-selectin
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 322 | 1820 | 5020 | 331 | 1849 | 5389 |
| Standard deviation | 15.63 | 121.94 | 256.02 | 21.18 | 144.22 | 301.78 |
| CV(%) | 4.7% | 6.7% | 5.6% | 6.4% | 7.8% | 5.6% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 322 | 316 | 323 | 357 | 329 | 16.1 | 4.8% |
| Sample 2 | 1820 | 1756 | 2051 | 1775 | 1850 | 118.06 | 6.3% |
| Sample 3 | 5020 | 5825 | 5171 | 5716 | 5433 | 343.86 | 6.3% |
*number of samples for each test n=16.
10ng/tube
