Description
Boster’s human MIP-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-1 alpha has been precoated onto 96-well plates. Standards(E.coli, A27-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MIP-1 alpha amount of sample captured in plate.
Synonyms: C C motif chemokine 3|CCL3|CCL3_HUMAN|Chemokine (C C motif) ligand 3|Chemokine ligand 3|G0/G1 switch regulatory protein 19 1|G0/G1 switch regulatory protein 19-1|G0S19 1|G0S19 1 protein|Heparin binding chemotaxis protein|L2G25B|LD78-alpha(4-69)|LD78ALPHA|Macrophage inflammatory protein 1 alpha|Macrophage inflammatory protein 1-alpha|MIP 1 alpha|MIP-1-alpha|MIP-1-alpha(4-69)|MIP1A|PAT 464.1|SCYA 3|SCYA3|SIS alpha|SIS beta|SIS-beta|Small inducible cytokine A3|Small-inducible cytokine A3|Tonsillar lymphocyte LD78 alpha protein
Species: Human MIP-1 alpha
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human MIP-1 alpha
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, A27-A92
Gene name: C-C motif chemokine 3 / chemokine (C-C motif) ligand 3
Protein name: C-C motif chemokine 3
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|33|175|370|30|166|361
Standard deviation|2.54|10.32|14.8|2.58|12.45|20.93
CV(%)|7.7%|5.9%|4%|8.6%|7.5%|5.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 34 | 32 | 35 | 33 | 1.11 | 3.3% |
| Sample 2 | 175 | 166 | 172 | 161 | 168 | 5.4 | 3.2% |
| Sample 3 | 370 | 407 | 390 | 394 | 390 | 13.27 | 3.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0448-CAP|96-well plate precoated with anti- human MIP-1 alpha antibody|1
EK0448-ST|Lyophilized recombinant human MIP-1 alpha standard|10ng/tubex2
EK0448-DA|Biotinylated anti- human MIP-1 alpha antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
