Description
Boster’s human MCP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for MCP-1 has been precoated onto 96-well plates. Standards(E.coli, 24-T99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MCP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MCP-1 amount of sample captured in plate.
Synonyms: C-C motif chemokine 2|_x000D_CCL2|_x000D_CCL2_HUMAN|_x000D__x000D__x000D_Chemokine (C-C motif) ligand 2|_x000D_GDCF-2|_x000D_HC11|_x000D_HSMCR30|_x000D_MCAF|_x000D_MCP-1|_x000D_MCP1|_x000D_MGC9434|_x000D_Monocyte chemoattractant protein 1|_x000D_Monocyte chemoattractant protein-1|_x000D_Monocyte chemotactic and activating factor|_x000D_Monocyte chemotactic protein 1|_x000D_Monocyte secretory protein JE|_x000D_SCYA2|_x000D_Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|_x000D_Small inducible cytokine subfamily A (Cys-Cys), member 2|_x000D_Small-inducible cytokine A2|_x000D_SMC-CF
Species: Human MCP-1
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human MCP-1
Cross Reactivity: There is cross-reactivity with human Eotaxin, MCP-3<1%. Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, Q24-T99
Gene name: C-C motif chemokine 2 / chemokine (C-C motif) ligand 2
Protein name: C-C motif chemokine 2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|31|159|477|30|148|481
Standard deviation|1.55|8.74|32.91|1.98|9.76|37.51
CV(%)|5%|5.5%|6.9%|6.6%|6.6%|7.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 33 | 37 | 35 | 34 | 2.23 | 6.5% |
| Sample 2 | 159 | 139 | 156 | 158 | 153 | 8.15 | 5.3% |
| Sample 3 | 477 | 421 | 423 | 456 | 444 | 23.46 | 5.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0441-CAP|96-well plate precoated with anti- human MCP-1 antibody|1
EK0441-ST|Lyophilized recombinant human MCP-1 standard|10ng/tubex2
EK0441-DA|Biotinylated anti- human MCP-1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
