Description
Boster’s human Marapsin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Marapsin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: M35-K290) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Marapsin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Marapsin amount of sample captured in plate.
Synonyms: CAPH2 | Marapsin | MPN | Pancreasin | Prss27 | Prss 27 | Protease serine 27 | UNQ1884/PRO4327 | Q9BQR3
Species: human Marapsin
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Marapsin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, M35-K290
Gene name: Serine protease 27 / protease, serine 27
Protein name: Serine protease 27
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|167|1395|5662|161|1417|5245
Standard deviation|10.35|58.59|311.41|12.39|77.93|325.19
CV(%)|6.2%|4.2%|5.5%|7.7%|5.5%|6.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 167 | 176 | 171 | 165 | 169 | 4.2 | 2.4% |
| Sample 2 | 1395 | 1278 | 1352 | 1276 | 1325 | 50.59 | 3.8% |
| Sample 3 | 5662 | 5287 | 5308 | 6068 | 5581 | 318.07 | 5.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1479-CAP|96-well plate precoated with anti- human Marapsin antibody|1
EK1479-ST|Lyophilized recombinant human Marapsin standard|10ng/tube×2
EK1479-DA|Biotinylated anti- human Marapsin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
