Description
Boster’s human LYVE-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LYVE-1 has been precoated onto 96-well plates. Standards(NSO, S24-T238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LYVE-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LYVE-1 amount of sample captured in plate.
Synonyms: cell surface retention sequence binding protein-1|_x000D_Cell surface retention sequence-binding protein 1|_x000D_CRSBP-1|_x000D__x000D__x000D_CRSBP1|_x000D_extracellular link domain containing 1|_x000D_extracellular link domain-containing 1|_x000D_Extracellular link domain-containing protein 1|_x000D_HAR|_x000D_Hyaluronic acid receptor|_x000D_lymphatic vessel endothelial hyaluronan receptor 1|_x000D_Lymphatic vessel endothelial hyaluronic acid receptor 1|_x000D_LYVE-1|_x000D_LYVE1|_x000D_LYVE1_HUMAN|_x000D_XLKD1
Species: Human LYVE-1
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human LYVE-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, S24-T238
Gene name: Lymphatic vessel endothelial hyaluronic acid receptor 1 / lymphatic vessel endothelial hyaluronan receptor 1
Protein name: Lymphatic vessel endothelial hyaluronic acid receptor 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|82|287|987|78|288|893
Standard deviation|4.59|19.22|52.31|5.85|19.87|63.4
CV(%)|5.6%|6.7%|5.3%|7.5%|6.9%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 82 | 86 | 81 | 71 | 80 | 5.52 | 6.9% |
| Sample 2 | 287 | 265 | 265 | 284 | 275 | 10.3 | 3.7% |
| Sample 3 | 987 | 829 | 988 | 991 | 948 | 69.15 | 7.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0967-CAP|96-well plate precoated with anti- human LYVE-1 antibody|1
EK0967-ST|Lyophilized recombinant human LYVE-1 standard|10ng/tube×2
EK0967-DA|Biotinylated anti- human LYVE-1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
