Human Lipocalin-2/NGAL PicoKine™ One Step ELISA Kit

Brand:
Boster Bio
Storage:

For the quantitation of Human LCN2 concentrations in cell culture supernates, serum, plasma(heparin), saliva and urine.   

 

Available on backorder

SKU: FEK0853 Category:

Description

The Boster One Step Picokine™ Human LCN2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human LCN2 with a 96-well strip plate that is pre-coated with antibody specific for LCN2. The detection antibody is a HRP conjugated antibody specific for LCN2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human LCN2 with immunogen: Expression system for standard: NSO; Immunogen sequence: Q21-G198. The kit is analytically validated with ready to use reagents.
To measure Human LCN2, add standards and samples to the wells, then add HRP conjugated detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human LCN2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Human LCN2 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster’s ELISA Resource Center at https://www.bosterbio.com/elisa-technical-resource-center.


Synonyms: LCN2; HNL; NGAL; Neutrophil gelatinase-associated lipocalin; NGAL; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin; p25

Species: Human LCN2

Assay: 156pg/ml-10000pg/ml

Sensitivy: <10pg/ml Specifity: Natural and recombinant Human LCN2

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat

Anticoagulant: heparin

Immunogen: Expression system for standard: NSO; Immunogen sequence: Q21-G198

Gene name: Neutrophil gelatinase-associated lipocalin / lipocalin 2

Protein name: Neutrophil gelatinase-associated lipocalin

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.(Shipped with wet ice.)


|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|285|1624|5541|290|1737|5384
Standard deviation|18.52|86.07|243.8|22.04|99|285.35
CV(%)|6.5%|5.3%|4.4%|7.6%|5.7%|5.3%


Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 285 255 236 267 253 15.43 6.1%
Sample 2 1624 1649 1587 1810 1667 120.02 7.2%
Sample 3 5541 5497 5323 4757 5279 306.18 5.8%

*number of samples for each test n=16.


10ng/tube