Human LILRA3 PicoKine ELISA Kit

Brand:
Boster Bio
Storage:

For quantitative detection of human LILRA3 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).   

 

Available on backorder

SKU: EK2113 Category:

Description

Boster’s Human LILRA3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for LILRA3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G24-E439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LILRA3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LILRA3 amount of sample captured in plate.


Synonyms: HM31; HM43; ILT6; LIR4; CD85E; ILT-6; LIR-4

Species: Human LILRA3

Assay: 312pg/ml-20000pg/ml

Sensitivy: <10pg/ml Specifity: Natural and recombinant human LILRA3

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat

Anticoagulant: heparin, EDTA or citrate

Immunogen: NSO, G24-E439

Gene name: Leukocyte immunoglobulin-like receptor subfamily A member 3 / leukocyte immunoglobulin like receptor A3

Protein name: Leukocyte immunoglobulin-like receptor subfamily A member 3

Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)


|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|534|2702|8939|578|2835|9176
Standard deviation|26.17|154.01|688.30|41.62|238.14|835.02
CV(%)|4.9%|5.7%|7.7%|7.2%|8.4%|9.1%


Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 534 575 581 544 559 31.86 5.7%
Sample 2 2702 2815 2838 2912 2817 174.65 6.2%
Sample 3 8939 9116 8672 9284 9003 783.26 8.7%

*number of samples for each test n=16.


Catalog number|Description|Quantity
EK2111-CAP|96-well plate precoated with anti- human LILRA3 antibody|1
EK2111-ST|Lyophilized recombinant human LILRA3 standard|20ng/tube×2
EK2122-DA|Biotinylated anti- human LILRA3 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4