Description
Boster’s human LAMA1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from rabbit specific for LAMA1 has been precoated onto 96-well plates. Standards(from human fibroblasts) and test samples are added to the wells, a biotinylated detection polyclonal antibody from rabbit specific for LAMA1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LAMA1 amount of sample captured in plate.
Synonyms: Lamb2|_x000D_LAMB2_HUMAN|_x000D_LAMC1|_x000D__x000D__x000D_LAMC2|_x000D_LAMC3|_x000D_laminin A|_x000D_Laminin B|_x000D_Laminin B1s chain|_x000D_Laminin gamma 1|_x000D_Laminin gamma 2|_x000D_Laminin gamma 3|_x000D_Laminin subunit beta-2|_x000D_Laminin-11 subunit beta|_x000D_Laminin-14 subunit beta|_x000D_Laminin-15 subunit beta|_x000D_Laminin-3 subunit beta|_x000D_Laminin-4 subunit beta|_x000D_Laminin-7 subunit beta|_x000D_Laminin-9 subunit beta|_x000D_OCCM|_x000D_S-LAM beta|_x000D_S-laminin subunit beta
Species: Human LAMA1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural human LAMA1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from rabbit|a biotinylated polyclonal antibody from rabbit
Anticoagulant: /
Immunogen: from human fibroblasts
Gene name: Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2 / laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S)
Protein name: Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|318|1051|4462|295|1015|4289
Standard deviation|23.21|47.29|348.03|26.25|58.87|411.74
CV(%)|7.3%|4.5%|7.8%|8.9%|5.8%|9.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 318 | 364 | 314 | 373 | 342 | 26.47 | 7.7% |
| Sample 2 | 1051 | 1242 | 1122 | 1215 | 1157 | 75.9 | 6.5% |
| Sample 3 | 4462 | 4109 | 4606 | 4544 | 4430 | 192.37 | 4.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0434-CAP|96-well plate precoated with anti- human LAMA1 antibody|1
EK0434-ST|Lyophilized human LAMA1 standard|10ng/tubex2
EK0434-DA|Biotinylated anti- human LAMA1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
