Description
Boster’s human KLK5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK5 has been precoated onto 96-well plates. Standards(NSO, V23-S293) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK5 amount of sample captured in plate.
Synonyms: Kallikrein 5|_x000D_Kallikrein-like protein 2|_x000D_kallikrein-related peptidase 5|_x000D__x000D__x000D_KLK L2|_x000D_SCTE|_x000D_Stratum corneum tryptic enzyme
Species: Human KLK5
Assay: 78pg/ml-5000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human KLK5
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, V23-S293
Gene name: Kallikrein-5 / kallikrein-related peptidase 5
Protein name: Kallikrein-5
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|134|809|2161|146|747|2368
Standard deviation|5.36|33.97|133.98|8.32|32.12|149.18
CV(%)|4%|4.2%|6.2%|5.7%|4.3%|6.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 134 | 128 | 140 | 148 | 137 | 7.39 | 5.3% |
| Sample 2 | 809 | 798 | 776 | 808 | 797 | 13.27 | 1.6% |
| Sample 3 | 2161 | 2628 | 2234 | 2197 | 2305 | 188.26 | 8.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0817-CAP|96-well plate precoated with anti- human KLK5 antibody|1
EK0817-ST|Lyophilized recombinant human KLK5 standard|10ng/tube×2
EK0817-DA|Biotinylated anti- human KLK5 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
