Description
Boster’s human KLK11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK11 has been precoated onto 96-well plates. Standards (NSO,E19-N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK11 amount of sample captured in plate.
Synonyms: Hippostasin|hK11|Kallikrein 11|Kallikrein 11 isoform 1 preproprotein|Kallikrein 11 isoform 2 precursor|Kallikrein 11 precursor| Kallikrein related peptidase 11|Kallikrein-11 inactive chain 2|KLK 11|Klk11|KLK11_HUMAN|MGC33060|Protease serine 20 trypsin like| Protease serine trypsin like|PRSS20|Serine protease 20|TLSP|Trypsin like protease|Trypsin-like protease
Species: human KLK11
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human KLK11
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E19-N250
Gene name: Kallikrein-11 / kallikrein-related peptidase 11
Protein name: Kallikrein-11
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|217|1054|4898|232|994|4808
Standard deviation|12.8|83.26|240|16.93|92.44|259.63
CV(%)|5.9%|7.9%|4.9%|7.3%|9.3%|5.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 217 | 206 | 233 | 215 | 217 | 9.73 | 4.4% |
| Sample 2 | 1054 | 1031 | 1100 | 1045 | 1057 | 25.86 | 2.4% |
| Sample 3 | 4898 | 4850 | 4518 | 5291 | 4889 | 274.24 | 5.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1166-CAP|96-well plate precoated with anti- human KLK11 antibody|1
EK1166-ST|Lyophilized recombinant human KLK11 standard|10ng/tube×2
EK1166-DA|Biotinylated anti- human KLK11 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
