Description
Boster’s human Ki67 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ki67 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ki67 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67; MKI67
Species: Human Ki67
Assay: 7.8pg/ml-500pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human Ki67
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen:
Gene name: Proliferation marker protein Ki-67 / marker of proliferation Ki-67
Protein name: Proliferation marker protein Ki-67
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|15|54|222|16|60|234
Standard deviation|0.9|3.13|10.4|0.94|4.2|18.25
CV(%)|6.3%|5.8%|4.7%|5.9%|7.0%|7.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 15 | 17 | 17 | 15 | 16 | 1.00 | 6.2% |
| Sample 2 | 54 | 64 | 64 | 54 | 59 | 5.00 | 8.4% |
| Sample 3 | 222 | 213 | 228 | 245 | 227 | 11.68 | 5.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1759-CAP|96-well plate precoated with anti- human Ki67 antibody|1
EK1759-ST|Lyophilized recombinant human Ki67 standard|1ng/tube×2
EK1759-DA|Biotinylated anti- human Ki67 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
