Description
Boster’s human IGFBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP4 has been precoated onto 96-well plates. Standards (NSO, D22-E258) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP4 amount of sample captured in plate.
Synonyms: AI875747|BP 4|BP4|Deb2|HT29 IGFBP|IBP 4|IBP-4|IBP4|IBP4_HUMAN|IGF binding protein 4|IGF-binding protein 4|IGF-BP4|IGFBP 4|IGFBP-4|
IGFBP4|Insulin Like Growth Factor Binding Protein 4|Insulin-like growth factor-binding protein 4
Species: Human IGFBP4
Assay: 500pg/ml-32000pg/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant human IGFBP4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, D22-E258
Gene name: Insulin-like growth factor-binding protein 4 / insulin-like growth factor binding protein 4
Protein name: Insulin-like growth factor-binding protein 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|634|3283|15689|630|3596|16628
Standard deviation|29.16|164.15|957.02|40.32|222.95|1330.24
CV(%)|4.6%|5%|6.1%|6.4%|6.2%|8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 634 | 669 | 599 | 583 | 621 | 33.16 | 5.3% |
| Sample 2 | 3283 | 3066 | 3571 | 3160 | 3270 | 190.05 | 5.8% |
| Sample 3 | 15689 | 15202 | 16042 | 14388 | 15330 | 620.39 | 4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0388-CAP|96-well plate precoated with anti- human IGFBP4 antibody|1
EK0388-ST|Lyophilized recombinant human IGFBP4 standard|32ng/tube×2
EK0388-DA|Biotinylated anti- human IGFBP4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
