Description
Boster’s human EGFR ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EGFR has been precoated onto 96-well plates. Standards(NSO, M1-S645) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGFR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EGFR amount of sample captured in plate.
Synonyms: 9030024J15Rik|AI552599|Avian erythroblastic leukemia viral (v erb b) oncogene homolog|Avian erythroblastic leukemia viral (verbb) oncogene homolog|Cell growth inhibiting protein 40|Cell proliferation inducing protein 61|EGF R|EGFR|EGFR_HUMAN|Epidermal growth factor receptor|Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)|Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian)Erbb|erbb 1|Erbb1|Erythroblastic leukemia viral (v erb-b) oncogene homolog|HER1|mENA|Oncogene ERBB|OTTMUSP00000005385|OTTMUSP00000005386|PIG61|Proto oncogene c ErbB 1|Proto-oncogene c-ErbB-1|Receptor tyrosine protein kinase ErbB 1|Receptor tyrosine protein kinase ErbB1|Receptor tyrosine-protein kinase ErbB-1|RP23-295E4.1|Urogastrone|wa2|Wa5
Species: Human EGFR
Assay: 156pg/ml-10000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human EGFR
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, M1-S645
Gene name: Epidermal growth factor receptor / epidermal growth factor receptor
Protein name: Epidermal growth factor receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|289|1634|5526|313|1731|5572
Standard deviation|13.58|81.7|408.92|18.78|102.12|417.9
CV(%)|4.7%|5%|7.4%|6%|5.9%|7.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 289 | 316 | 285 | 279 | 292 | 14.16 | 4.8% |
| Sample 2 | 1634 | 1690 | 1737 | 1686 | 1686 | 36.46 | 2.1% |
| Sample 3 | 5526 | 5048 | 5070 | 5056 | 5175 | 202.8 | 3.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0327-CAP|96-well plate precoated with anti- human EGFR antibody|1
EK0327-ST|Lyophilized recombinant human EGFR standard|10ng/tube×2
EK0327-DA|Biotinylated anti- human EGFR antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
