Description
Boster’s human Decorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Decorin has been precoated onto 96-well plates. Standards(sf21, G17-K359) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Decorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Decorin amount of sample captured in plate.
Synonyms: Bone proteoglycan II|_x000D_CSCD|_x000D_DCN|_x000D__x000D__x000D_DCN protein|_x000D_Decorin|_x000D_Decorin proteoglycan|_x000D_Dermatan sulphate proteoglycans II|_x000D_DKFZp686J19238|_x000D_DSPG 2|_x000D_DSPG2|_x000D_PG 40 |_x000D_PG II|_x000D_PG S2|_x000D_PG-S2|_x000D_PG40|_x000D_PGII|_x000D_PGS 2|_x000D_PGS2|_x000D_PGS2_HUMAN|_x000D_Proteoglycan core protein|_x000D_SLRR1B|_x000D_Small leucine rich protein 1B
Species: Human Decorin
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Decorin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated monoclonal antibody from mouse
Anticoagulant: heparin or EDTA
Immunogen: sf21, G17-K359
Gene name: Decorin / decorin
Protein name: Decorin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|60|247|1001|56|270|1067
Standard deviation|4.56|16.54|70.07|4.31|20.25|88.56
CV(%)|7.6%|6.7%|7%|7.7%|7.5%|8.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 51 | 50 | 59 | 55 | 4.52 | 8.2% |
| Sample 2 | 247 | 279 | 302 | 287 | 278 | 20.1 | 7.2% |
| Sample 3 | 1001 | 962 | 922 | 976 | 965 | 28.61 | 2.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0749-CAP|96-well plate precoated with anti- human Decorin antibody|1
EK0749-ST|Lyophilized recombinant human Decorin standard|10ng/tube×2
EK0749-DA|Biotinylated anti- human Decorin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
